Time-lapse experiments showing deltorphin II (100 nM; a selective agonist)-induced internalization of delta-opioid receptor–enhanced GFP (DOR-EGFP; green) receptors expressed in HEK 293 cells. The C-terminal DOR-EGFP fusion shows unchanged binding, signaling, internalization and down-regulation properties when expressed in HEK-293 cells.
Representative experiment is shown. Samples were observed under a Leica confocal microscope (SP2 AOBS MP) with objective 63X at 37°C. Images were recorded automatically every 30 s during 60 min.
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