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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34538*  Cite 
Description

Movie showing motility and morphology of cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) arrested in G1 using 0.08 uM nocodazole (Uetake and Sluder 2010, Curr Biol 20:1666-1671). The time series used to create the movie is gropued in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image 2.0.1.11 software.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
retinal pigment epithelium
Cell Line
hTERT-RPE1
Biological Context
Biological Process
cell motility
cell morphology
Attribution
Names
Yumi Uetake
Greenfield Sluder
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34538
Archival Resource Key (ARK)
ark:/b7295/w9cil34538
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.645µm
Y 1024px 0.645µm
Time 180 seconds 1868