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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34925*  Cite 
Description

Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 (Arp2/3) primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal Arp2/3 complex at Y-junctions at cell edge. Fluorescence microscopy and corresponding intensity profiles double stained with TRITC-phalloidin (red) and p21 antibodies (green) is available at CIL 34922. Image corresponds to Figure 5d from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
fibroblast
Cellular Component
actin cytoskeleton
Arp2/3 protein complex
cell edge
Biological Context
Biological Process
branching of actin filaments
cytochalasin D treatment
actin filament organization
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34925
Archival Resource Key (ARK)
ark:/b7295/w9cil34925
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
primary antibody plus labeled secondary antibody
immuno-gold
Processing History
pseudocolored
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 780px 2.75nm
Y 659px 2.75nm