Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 (Arp2/3) primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal Arp2/3 complex at Y-junctions at cell edge. Fluorescence microscopy and corresponding intensity profiles double stained with TRITC-phalloidin (red) and p21 antibodies (green) is available at CIL 34922. Image corresponds to Figure 5d from J Cell Biol. 1999 May 31;145(5):1009-26.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
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