Primary bone marrow macrophages derived from mouse plated on a coverslip and imaged live. Phase contrast microscopy allows us to see the dense f-actin rich leading edge, ruffles, mitochondria, the nucleus, and other organelles throughout the volume of the cells. At the center of the image is a polarized lamellipod of a crawling cell. The glass micropipette at the bottom of the image is delivering CSF (colony stimulating factor) locally and the cell may be responding.
Live bone marrow macrophages plated in MatTek dish and imaged with a 60X N.A. 1.4 phase 3 Olympus objective on an IX71 microscope with a Cooke Sensicam QE camera and IPLab software running on a Dell Windows XP computer.
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