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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35128*  Cite 

Primary bone marrow macrophages derived from mouse plated on a coverslip and imaged live. Internal Reflection Microscopy (IRM) shows direct apposition of the cells to coverslip with very little depth of field. Note the light gray uniform background extends under the cells' footprints demonstrating that the cells do not adhere tightly to the substrate. At the center of the image is a large polarized lamellipod of a crawling cell. The small spot below the cell is the tip of glass micropipette delivering CSF (colony stimulating factor) locally and the cell may be responding.

Technical Details

Live bone marrow macrophages plated in MatTek dish and imaged with a 60X N.A. 1.4 phase 3 Olympus objective on an IX71 microscope with a Cooke Sensicam QE camera and IPLab software running on a Dell Windows XP computer. The reflection microscopy was performed by removing the emission filter from a standard narrow pass rhodamine filter cube. Light was at approximately 570 nm and reflection leaked through the dichroic to allow for imaging.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Bone marrow
Cellular Component
ruffle membrane
Biological Context
Biological Process
ruffle organization
Molecular Function
chemokine receptor activity
Mouse Gross Anatomy
Michael Cammer
Dianne Cox
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
interference reflection contrast (IRM)
Parameters Imaged
interference reflection contrast (IRM)
Source of Contrast
interference contrast
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1376px 0.11µm
Y 1040px 0.11µm