Living Lilium pollen tube labeled with mitotracker green to mark mitochondria (green) and cytochalasin D TRITC to mark endoplasmic reticulum (red) imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.
After labeling, living lily pollen tubes were imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green and red signals from a central x-y plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.
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