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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35284*  Cite 
Description

Living Lilium pollen tube labeled with mitotracker green to mark mitochondria (green) and cytochalasin D TRITC to mark endoplasmic reticulum (red) imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.

Technical Details

After labeling, living lily pollen tubes were imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green and red signals from a central x-y plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
mitochondria
endoplasmic reticulum
Biological Context
Biological Process
pollen tube growth
Attribution
Names
Alenka Lovy-Wheeler
Luis Cardenas
Joseph Kunkel
Peter K Hepler
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35284
Archival Resource Key (ARK)
ark:/b7295/w9cil35284
Grouping This image is part of a group.
Imaging
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
mitotracker green
Tetramethyl rhodamine (TRITC)
Processing History
living tissue
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 492px ——
Y 147px ——