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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35287*  Cite 

Fixed Lilium pollen tubes were treated with fluorescent phalloidin to label filamentous actin, and confocal images obtained. A strongly labeled actin fringe near the growing tip is present. Other images in the group reveal the distributions of additional cytoskeletal elements and membranous components.

Technical Details

Germinated lily pollen was fixed with paraformaldehyde and glutaraldehye, treated with Alexa-543 phalloidin to label actin, and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Image is a projection of several confocal x-y slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
actin filament
Biological Context
Biological Process
pollen tube growth
Alenka Lovy-Wheeler
Kathleen L Wilsen
Tobias I Baskin
Peter K Hepler
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
alexa fluor 543
Processing History
projection of slices
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 403px ——
Y 87px ——