Living Lilium pollen tube labeled with mGFP5-HDEL to mark endoplasmic reticulum (green) imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.
Living lily pollen tubes were labeled by bombardment with tungsten bullets coated with plasmid DNA containing the mGFP5-HDEL gene and, after expression, imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The signal from a central x-y plane through the tube is shown. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.
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