Distribution of Nup133, Nup43 and Nup62 (green) and tubulin (blue) in spindles assembled in vitro from Xenopus oocyte extracts. Chromosomes are stained with DAPI (blue). See Orjalo et al 2006. Mol Biol Cell 17:3806-3808.
Xenopus egg extracts were mixed with sperm chromatin, treated to induce spindle formation, antibodies added, and the preparations fixed with paraformaldehyde, and centrifuged onto cover slips through a glycerol cushion, and processed for immunofluorescence. z-stacks of images were recorded using a Zeiss Axioskop microscope with 63x !.4 NA objective lens. See Fig 3A in Orjalo et al. 2006 Mol Biol Cell 17:3806-3818.
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