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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35645*  Cite 

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc20, a cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C) required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate novel protein thought to be involved in cell cycle regulation. These phase, gfp images are part of an image group that ranges from CIL:35640-35659. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
CDC20 protein
mitotic checkpoint complex
anaphase-promoting complex
David Ball
Jean Peccoud
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
phase contrast microscopy
Parameters Imaged
fluorescence emission
elastic scattering of photons
Source of Contrast
distribution of a specific protein
differences in amount of elastic light scattering
Visualization Methods
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Time 300 seconds 61