The deep fiber is associated with the cytopharyngeal membrane (see Smith and Bunse, Trans. Am. Microsc. Soc. 102:264-271, 1983). This membrane has acidosome-like vesicles docked at its cytosolic surface. Enlargement of this membrane tube probably permits new vacuole formation. The deep fiber is probably homologous to the postoral microtubular bundles of Paramecium. TEM taken on 2/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 11,250X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39725) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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