The contractile vacuole pore is supported on its cytosolic side by a coil of microtubules and other microtubules extend from the pore to the CV membrane. The plasma membrane and CV membrane cover the bottom of the pore. Bacteria reside in the chamber. TEM taken on 2/14/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39505) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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