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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36542*  Cite 
Description

This image is part of a large data set of Xenopus laevis eggs imaged at various times post fertilization (the first number of the file name corresponds minutes, eg 30_2 is 30 min post fertilization). The embryos are stained for alpha-tubulin (green) using a directly labeled B-5-1-2 antibody and DNA (red).

Technical Details

Images collected on a Zeiss LSM 710 confocal with a 10X 0.3 NA objective using 561 and 633 laser lines.

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
fertilized egg
Cellular Component
microtubule
DNA
Biological Context
Biological Process
first cell cycle
Attribution
Names
Martin Wuehr
Timothy Mitchison
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36542
Archival Resource Key (ARK)
ark:/b7295/w9cil36542
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
distribution of a specific protein
Visualization Methods
labeled primary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2592px 0.48µm
Y 2592px 0.48µm