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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36684*  Cite 

Following discharge, remnant trichocyst membrane does not appear to directly enter the trans Golgi network for reuse in new trichocyst formation. Rather the HRP will be transferred to lysosomes (not shown) so the membrane presumably becomes part of the lysosomal membrane and eventually part of the phagolysosome digestive vacuole. The HRP content will be subjected to digestion. Exactly what happens to all the excess membrane is unknown although it probably becomes part of the cell’s overall membrane pool which must be continuously synthesized and replenished as it is halved at each cell division that occurs at 24 hour intervals. TEM taken on 4/12/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µ

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
organelle membrane
Golgi trans cisterna
Biological Context
Biological Process
organelle assembly
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Sample Preparation
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 2118px ——
Y 2538px ——