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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36696*  Cite 
Description

The buccal cavity opens into the developing digestive (food) vacuole at the terminal edges of the alveolar sacs that cover the buccal cavity. A plane connecting these edges would roughly describe a tear drop-shaped opening that has been called the cytostome or cell mouth. The cytopharynx (cp) just beyond the buccal cavity encircles the cytostome. The cytopharynx consists of two sides, the left side (shown here) from which 40 or more ribbons of cytopharyngeal microtubules curve over a cytostomal cord. This edge runs along the dorsal side of the quadrulus and borders the terminal margins of the alveolar sacs. Discoidal vesicles are transported along the microtubular ribbons to the left edge of the cytopharynx. TEM taken on 3/25/81 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
oral apparatus
food vacuole
Biological Context
Biological Process
oral apparatus organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36696
Archival Resource Key (ARK)
ark:/b7295/w9cil36696
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 3136px ——
Y 3606px ——