High magnification view of the curved cytopharyngeal microtubular ribbons as they pass over the cytostomal cord. They carry the discoidal vesicles to their fusion sites at the cytopharyngeal membrane. Chemical fixation results in the loss of their tight association with the anterior sides of the ribbons as can be seen in quick frozen cells. Acidosomes seem to dock with the nascent vacuole and leave the microtubular ribbon as soon as they make contact with the vacuole membrane. Sections of finger-like extensions of the alveoli should not be confused with carrier vesicles. TEM taken on 3/25/81 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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