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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36729*  Cite 

Higher magnification of acidosomes docked at the nascent digestive vacuole membrane. The E-fracture face is relatively IMP free while the P-fracture face has many large IMPs as well as some sizable pits where chunks of this leaflet seem to have stuck to the E-face. Tiny pits on the E-face indicate the removal of individual IMPs from this leaflet. An indentation of the membrane into the lumen of the acidosome shows IMPs on its exposed P-face; which, if any, of these IMPs are V-ATPases is not known. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.

Technical Details

A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40560) which is available for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
early phagosome
vesicle membrane
oral apparatus
Biological Context
Biological Process
membrane docking
phagosome acidification
digestive system process
Richard Allen (University of Hawaii)
J Cell Sci. 1993 Sep;106 ( Pt 1):411-22.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
shadowing and plating
recorded image
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2142px ——
Y 2802px ——