Secondary lysosomes dock only with phagoacidosome membrane at a distance of 40nm. As during acidosome fusion with phagosomes, fusion of the many lysosomes with the phagoacidosome occurs at nearly the same time. Some small vesicles can be found close to the lysosomal surface. Such small vesicles might represent primary lysosomes originating from Golgi complexes, such as the coated vesicle in the upper left corner of this micrograph might suggest. TEM taken on 8/16/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 31,500X. Bar = 0.2µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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