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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36742*  Cite 

Acid phosphatase is synthesized in the biosynthetic pathway in the ER and packaged in the TGN into primary lysosomes which are passed on to secondary lysosomes. Primary lysosomes are small vesicles of 75-100nm filled with acid phosphatase and no substrate. They probably fuse with secondary lysosomes before the secondary lysosomes fuse with the DV-II. Small vesicles of the size of primary lysosomes are near secondary lysosomes. TEM taken on 3/26/79 by J. Muraoka with Hitachi HU11A operating at 75kV. Neg. 25,500X. Bar = 0.2µm.

Technical Details

Glutaraldehyde fixation followed by Gomori Staining, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
primary lysosome
Golgi stack
endoplasmic reticulum
Biological Context
Biological Process
protein targeting to Golgi
acid phospatase localization
Richard Allen (University of Hawaii)
J. Muraoka
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2220px ——
Y 2490px ——