The entire membrane of the phagolysosome does appear to have been changed into that similar to the lysosome membrane which bears a thick glycocalyx (polysaccharide coat) and no longer appears to be like the phagoacidosome which does not have a thick glycocalyx. As no patches of acidosome-like membrane remain it can be assumed that the acidosome-like membrane has been removed. Fibrous material lines the cytosolic side of the early DV-III similar to that found at docking sites between the lysosomes and the DV-II. TEM taken on 3/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. Bar = 0.2µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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