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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37141*  Cite 
Description

Transmission electron micrograph of exocytosis taken from the rat pancreas. Images such as these indicated that secretory granules closely and selectively approach the apical membrane of the pancreatic acinar cell where exocytosis takes place, fuse with the apposed membrane, and release the content of secretory proteins into the acinar lumen without broaching the intracellular environment. The resulting omega figure is transitory, suggesting that the excess granule membrane is retrieved/recycled. Note the actin coat around the newly inserted granule membrane. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Additional Reference: Valentijn, K.M., F.D. Gumkowski and J.D. Jamieson. 1999. J. Cell Science. 112:81-96. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 33,000X.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
pancreatic acinar cell
Cellular Component
secretory granule
secretory granule membrane
Biological Context
Biological Process
exocytosis
Attribution
Names
George E. Palade
Link
George E. Palade EM SLide Collection
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37141
Archival Resource Key (ARK)
ark:/b7295/w9cil37141
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 4709px ——