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CIL:37152*  Cite 

Transmission electron micrograph of spectrin in cell fractionation. Dr. V. T. Marchesi and colleagues took advantage of the high purity of red cell ghosts obtained by hypotonic lysis to analyze the properties of membrane proteins. They identified spectrin as a major red cell membrane cytoskeletal protein responsible for maintaining the biconcave shape of red cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x25,000. Relevant citations: Marchesi, V.T. and G.E. Palade. 1967. J. Cell Biol. 35:385-404. Nicoloson, G.L., V.T. Marchesi and S.J. Singer. 1971. J. Cell Biol. 51:265-272. Marchesi, V.T. and E. Steers, Jr. 1968. Science. 159:203-204.

Biological Sources
Cell Type
blood cell
red blood cell
Cellular Component
plasma membrane
George E. Palade
Vincent. T. Marchesi
George E. Paldae EM Slide Collection
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
ferratin conjugated antibody
Sample Preparation
hypotonic lysis
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 4630px ——