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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37261*  Cite 
Description

Zymogen granule fraction Calf. This study demonstrated directly that the enzyme and proenzyme composition of proteins in pancreatic juice collected from an indwelling catheter in the bovine pancreatic duct correlated exactly with the protein composition obtained from lysed purified bovine zymogen granules. Subsequent studies showed that pancreatic digestive enzymes and proenzymes were secreted from the pancreas in parallel. In other words, each granule must contain, on average, the same composition of secretory proteins. This hypothesis was later directly tested by immuno-EM localization of trypsinogen and other secretory proteins in all zymogen granules in the bovine pancreas. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x2,000.

Biological Sources
NCBI Organism Classification
Bos taurus
Cellular Component
zymogen granule
Attribution
Names
George E. Palade
L. J. Greene
Published
JCB. 238:2054-2070, 1963
Pubmed
13950163
Link
George E. Palade EM Slide Collection
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37261
Archival Resource Key (ARK)
ark:/b7295/w9cil37261
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
lead salt
Processing History
scanned negative
Sample Preparation
Methods
osmium tetroxide fixed tissue
n-butyl methacrylate embedded tissue
Relation To Intact Cell
microtome-sectioned tissue