Transmission electron micrograph of rough microsomes obtained by fraction of guinea pig pancreas. Cell fractionation confirmed that newly synthesized acinar cell secretory proteins moved from the rough endoplasmic reticulum to the periphery of the Golgi in the lumen of a smooth-surfaced membrane bounded compartment. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
Additional technical details regarding cell fractionation, specimen processing, and electron microscopy can be found in: Jamieson, J.D., and Palade, G.E. J. Cell Biol. 34:577-596, 1967. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x10,000
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