CD3 mutant wheat (deficient in chlorophyll b) was germinated and grown for 6 days in the dark, greened at high light intensity, thylakoids prepared, plunge-frozen, and freeze-fracture replicas obtained and examined by transmission electron microscopy. This region shows the characteristic particle size and distribution found on the stacked (EFs, PFs) unstacked (EFu, PFu) membrane faces of stacked thylakoid membranes. Compared to the wildtype, there is increased density of particles on the EFs and EFu faces and decreased density on the PFs faces. This set of grouped images, which also shows thin sections, includes images of wild-type wheat.
Thylakoid preparations were equilibrated with 35% glycerol, plunge frozen in liquid Freon 12 cooled in liquid N2 and fractured and replicated in a Balzers BA 360 freeze etch device. Replicas were examined in a JEOL 100C at 80KV and images recorded on film. See Fig 9 in K D Allen et al. 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919.
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