Freeze-fracture image of a chloroplast from barley (Hordeum vulgare) illustrating the characteristic size and distribution of particles on the EFu and EFs thylakoid membrane faces. The inset gives a higher magnification view of the particles on the stacked ES face. Other images in this group show the changes in particle distribution in a pale green mutant that lacks chlorophyll b.
Wildtype and mutant plants were were grown under a 16-hr day regime, harvested at 3 weeks, and chloroplasts isolated. For freeze fracture, isolated chloroplasts were made 30% in glycerol, plunge frozen, and cleaved, etched, and shadowed in a Balzers freeze-fracture unit. Replicas were examined with a Philips 300 transmission EM and images recorded on film. See Fig 11 in KR Miller et al. 1976 J. Cell Biol 71, 624-638.
|Spatial Axis||Image Size||Pixel Size|