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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39115*  Cite 
Description

Freeze-fracture image of a chloroplast from barley (Hordeum vulgare) illustrating the characteristic size and distribution of particles on the EFu and EFs thylakoid membrane faces. The inset gives a higher magnification view of the particles on the stacked ES face. Other images in this group show the changes in particle distribution in a pale green mutant that lacks chlorophyll b.

Technical Details

Wildtype and mutant plants were were grown under a 16-hr day regime, harvested at 3 weeks, and chloroplasts isolated. For freeze fracture, isolated chloroplasts were made 30% in glycerol, plunge frozen, and cleaved, etched, and shadowed in a Balzers freeze-fracture unit. Replicas were examined with a Philips 300 transmission EM and images recorded on film. See Fig 11 in KR Miller et al. 1976 J. Cell Biol 71, 624-638.

Biological Sources
NCBI Organism Classification
Hordeum vulgare subsp. vulgare
Cell Type
leaf
Cellular Component
chloroplast
plastid thylakoid
photosystem II
Biological Context
Biological Process
C4 photosynthesis
Attribution
Names
Kenneth R Miller
Gayle J Miller
Katherine R McIntyre
Published
KR Miller, GJ Miller, KR McIntyre 1976 The light-harvesting chlrophyll-protein complex of photosystem II
Pubmed
993264
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39115
Archival Resource Key (ARK)
ark:/b7295/w9cil39115
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Data Qualifiers
processed data
Sample Preparation
Methods
freeze_fracture/freeze_etch
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 1248px ——
Y 1200px ——