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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39129*  Cite 
Description

The highest concentration of parasomal sacs is found in the oral region associated with the three ciliary membranelles, called in Paramecium, the ventral amd dorsal peniculi and the quadrulus. This micrograph shows the four rows each of the two peniculi, dorsal and ventral, and a large accumulation of coated vesicles and early endosomal cisternae immediately below the basal bodies. Two rows of parasomal sacs border the closely packed peniculi and a third row separates the two peniculi. Four rows of parasomal sacs interdigitate with the 4 rows of more loosely associated cilia in the quadrulus. TEM taken on 1/25/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
coated pit
cell cortex
mitochondrion
glycogen granule
Biological Context
Biological Process
cortical cytoskeleton organization
cytoplasm organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39129
Archival Resource Key (ARK)
ark:/b7295/w9cil39129
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4527px 0.77nm
Y 5231px 0.77nm