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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39145*  Cite 
Description

Cells of the symbiotic prochlorophyte Prochloron didemni were separated from its ascidian partner, thylakoids isolated, treated to liberate chlorophyll-containing complexes, and negatively stained preparations observed by transmission EM. The image shows an averaged top view of a chlorophyll-photsystem II super complex with overlays illustrating the putative locations of the core dimer and surrounding CP43 proteins obtained from x-ray analysis data. Chlorophyll is shown in green. Other images in this group illustrate other aspects of the structure and the overview shown in CIL 39146 illustrates the range of particle types in the preparation.

Technical Details

Fractions isolated from thylakoids were negatively stained with 2% uranyl acetate and observed with a Philips CM100 transmission EM at a magnification of 51,500x, images recorded on film, and digitized using a Leafscan densitometer yielding a pixel size after binning of 0.388 nm. Sub-populations were identified and class averages obtained using IMAGIC-5. See Fig 5 in TS Bibby, J Nield, M Chen, AWD Larkum, J Barber 2003 Structure of a photosystem II supercomplex isolated from Prochloron didemni retaining its chlorophyll a/b light-harvesting system. Proc Natl Acad Sci USA 100:9050-9054.

Biological Sources
NCBI Organism Classification
Prochloron sp.
Cellular Component
photosystem II
photosystem I
Biological Context
Biological Process
photosynthesis, light harvesting
Attribution
Names
Thomas S. Bibby
Jon Nield
Min Chen
Anthony W D Larkum
James Barber
Published
TS Bibby, J Nield, M Chen, AWD Larkum, J Barber 2003 Structure of a photosystem II supercomplex isolated from Prochloron didemni retaining its chlorophyll a/b light-harvesting system. Proc Natl Acad Sci USA 100:9050-9054
Pubmed
12837938
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39145
Archival Resource Key (ARK)
ark:/b7295/w9cil39145
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
montage
Data Qualifiers
processed data
Sample Preparation
Methods
negative stain
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 656px 0.388nm
Y 654px 0.388nm