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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39179^* Cite

Description

Carrier vesicles are around 100nm in diameter. They can carpet the cytopharyngeal ribbons with a layer of vesicles, several vesicles wide, as shown in this micrograph. As seen here the ribbons come to lie close to the source of many of these carrier vesicles that arise from early endosomes under the quadrulus. TEM taken on 5/16/73 by R. Allen with Hitachi HU11A TEM. Neg. 12,750X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus; cytoplasmic microtubule; carrier vesicles

Biological Context

Biological Process: oral apparatus organization; phagocytosis

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39179
Archival Resource Key (ARK): ark:/b7295/w9cil39179

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4702px	1.2nm
Y	5221px	1.2nm