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Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39240^* Cite

Description

A view of the locomotor ciliature of Conchophthirus. This includes the nature of the cell membrane and axoneme. The negative magnification is 22,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

Biological Sources

NCBI Organism Classification: uncultured scuticociliate; Conchophthirus curtus
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cilium axoneme; plasma membrane

Biological Context

Biological Process: cilium axoneme assembly

Attribution

Names: Gregory Antipa (San Francisco State University)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39240
Archival Resource Key (ARK): ark:/b7295/w9cil39240

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3853px	0.9nm
Y	3756px	0.9nm