Nuclear DNA was prepared from synchronously replicating nuclei of Physarum polycephalum, mixed with cytochrome c and formamide, and spread on the surface of a hypophase consisting of 10 mM Tris, 1 mM EDTA and 10% formamide. The surface containing the DNA-cytochrome c complexes was picked up on carbon-formvar grids, stained with ethanolic phosphotungstic acid, and rotary shadowed with Pt/Pd prior to TEM examination. Arrows point to replication forks.
See Fig 3A in: F Haugli et al. 1982 DNA replication in Physarum polycephalum: electron microscopic analysis patterns of DNA replication in the presence of cycloheximide. J Cell Biol 95:323-331.
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