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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39574*  Cite 
Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged SIC1. SIC1 is an inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 These phase, gfp images are part of an image group that ranges from CIL:39751-39755. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
SIC1 Protein
Attribution
Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39574
Archival Resource Key (ARK)
ark:/b7295/w9cil39574
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Z 21px 0.5µm
Time 300 microns 61