Alternate header for print version

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39673*  Cite 

10-hour timelapse of the double mutant Clb2dbΔ, Clb5Δ, which has the destruction box region of the CLB2 gene, on the cell cycle control system in budding yeast. The yeast, Saccharomyces cerevisia, are growing in 2% raffinose, since this mutation is inviable in glucose. The images from two experiments done with Wild-type Saccharomyces cerevisiae as a control are available as CIL:39606-39625 and CIL:39646-39665. The images from two experiments done with the double mutant Clb2dbΔ, Clb5Δ are available as CIL:39626-39645 and CIL:39666-39685.

Technical Details

Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage, automatic focusing, motorized illumination shutters and a CoolSNAP_HQ2 camera.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
cell surface
Biological Context
Biological Process
cell cycle checkpoint
cell budding
regulation of cell budding
Clb2dbΔ, Clb5Δ mutation
David Ball
Jean Peccoud
Cell Cycle 10:6, 999-1009; March 15, 2011;
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 0.213µm
Y 512px 0.213µm
Time 240 seconds 10 hr