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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39730*  Cite 
Description

TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were compacted in the appropriate salt, and mildly crosslinked with formaldehyde to preserve nucleosome-nucleosome interactions, then allowed to decondense in low salt prior to fixation with glutaraldehyde, and shadowing with Pt. (B-D with linker histone and without Mg; J-M) with linker histone and 1 mM MgCl2; (N-S)with linker histone and 4mM MgCl2. The drawings at right show the authors' interpretation of the adjacent micrograph showing crosslinked nucleosomes and the number of nucleosomes included in the 'loop' created by the crosslink. See Fig 3 in S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322 See Fig 1 in: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322

Technical Details

Reconstitutes were fixed in the appropriate salt environment, applied to thin carbon films, air dried, and shadowed with Pt using electron bean evaporation (Balzers BA 080 vacuum evaporator. Grids were examined in a FEI Tecnai 12 TEM at 100KV with a LaB6 filament and tilted darkfield optics, and recorded using a TVIPS 2k CCD camera. See also: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322.

Biological Sources
Cellular Component
chromatin
nucleosome
Biological Context
Biological Process
DNA packaging
chromatin organization
Attribution
Published
S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322
Pubmed
19651606
Link
Proceedings National Academy of Sciences USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39730
Archival Resource Key (ARK)
ark:/b7295/w9cil39730
Grouping This image is part of a group.
Imaging
Image Type
CCD
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 495px ——
Y 420px ——