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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39779*  Cite 

Transmission electron micrograph of a highly magnified and cropped image of a capillary in a mouse cardiac muscle. The capillary is used mainly for the transport of nutrients and oxygen through the elaborate network of branches. Made up of endothelial cells the capillary has the ability to constrict or dilate when signaled by the body. Red blood cells average about 7.7 um in diameter and seen here as a darkly colored region in which its’ sole function is to deliver oxygen throughout the tissues of the body.

Technical Details

Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
capillary endothelial cell
blood cell
Dee Lauzon
Sue Lancelle
Marian Rice
Mount Holyoke College
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Sample Preparation
glutaraldehyde fixed tissue
formaldehyde fixed tissue
ethanol dehydration
tissue in epoxy resin embedment
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 1805px ——
Y 1843px ——