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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39780*  Cite 

Transmission electron micrograph of ventricle tissue of a mouse cardiac muscle. This image shows the various areas of a muscle fiber. The Z lines are the very dark borders that link sarcomeres. The area between the Z lines is further divided into two lighter colored bands at either end called the I-bands, and a darker, grayish band in the middle called the A-band. The A band appears darker than the I band. The H zone is the bright region in the A band where there is no overlapping actin and myosin when the muscle is in the relaxed state. There are also many darkly stained mitochondria in this image.

Technical Details

Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cellular Component
Z disc
I band
A band
H zone
Biological Context
Biological Process
muscle contraction
Mouse Gross Anatomy
Dee Lauzon
Sue Lancelle
Marian Rice
Mount Holyoke College
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Sample Preparation
glutaraldehyde fixed tissue
formaldehyde fixed tissue
ethanol dehydration
tissue in epoxy resin embedment
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 1805px ——
Y 1810px ——