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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39791*  Cite 

Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. This high magnification image of the embryo shows the primary mesenchyme syncytial ring, where it attaches to the outer epithelial layer (ectoderm layer). The fine fibers are part of the blastocoel matrix. The primary mesenchyme syncytial ring is the precursor to spicule formation (developing calcareous larval skeleton). This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.

Technical Details

Images taken on a JEOL 35C SEM. Processing protocol: 1. 2% GTA in 75% MBL SW or FilteredSW FSW - fix for 1.5hrs. RT. 2. Rinse with 0.1M NaCacodylate buffer pH 7.4 3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold 4. Rinse in 0.1M NaCacodylate buffer ph 7.4 5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each 6. 100% - 3 changes - 15' each 7. Critical Point Dry 8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point). 9. Sputter coat with AuPd.

Biological Sources
NCBI Organism Classification
Lytechinus pictus
Cell Type
mesenchymal cell
epithelial cell
Cellular Component
extracellular matrix
extracellular region
Biological Context
Biological Process
embryonic morphogenesis
Louisa Howard
Dartmouth EM Facility
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
glutaraldehyde fixed tissue
ethanol dehydration
critical_point dried specimen
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 640px ——
Y 480px ——