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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39797*  Cite 
Description

Up to three basal bodies have been seen in various stages of development near a mature basal body/cilium complex. In this micrograph 2 new basal bodies are present next to 2 cilia and 3 are forming next to the third complex. Developing basal bodies can reorient parallel to the older basal body before it has elongated completely. TEM taken on 8/14/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Tetrahymena pyriformis
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
microtubule basal body
microtubule associated complex
Biological Context
Biological Process
microtubule basal body duplication
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39797
Archival Resource Key (ARK)
ark:/b7295/w9cil39797
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 6219px 0.78nm
Y 4960px 0.78nm