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CIL:39958*  Cite 

Postsynaptic densities (PSDs) in the CA1 region of the rat hippocampus, revealed by ethanolic phosphotungstic acid (EPTA) staining, were reconstructed from a maximum intensity projection of tomographic reconstruction (see, for example the blue arrow). Intensity was reversed so that PSDs appear bright against a dark background. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Martone M. E.; Jones Y. Z.; Young S. J. Ellisman; M. H. Zivin; J. A. and Hu B. R. (1999) Modification of postsynaptic densities following transient cerebral ischemia:a quantitative and three dimensional ultrastructural study. J. Neurosci.19:1988-97.

Technical Details

Electron microscopic methods: Tissue sections from were stained either by 1% ethanolic phosphotungstic acid (E-PTA) (Bloom and Aghajanian, 1966, 1968) or by the conventional osmium-uranium-lead method. Briefly, coronal brain sections were cut at a thickness of 200 um with a Vibratome through the level of the dorsal hippocampus and post-fixed for 1 hr with 4% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4. For conventional osmium-uranium-lead staining, sections were post-fixed for 2 hr in 1% osmium tetroxide in 0.1 M cacodylate buffer, rinsed in distilled water, and stained with 1% aqueous uranyl acetate overnight. The tissue sections were then dehydrated in an ascending series of ethanol to 100%, followed by dry acetone, and embedded in Durupan ACM. Thin sections were counterstained with lead citrate before examination in the electron microscope. For E-PTA staining, sections were dehydrated in an ascending series of ethanol to 100% and stained for 1 hr with 1% PTA prepared by dissolving 0.1 gm of PTA in 10 ml of 100% ethanol and adding four drops of 95% ethanol. Sections were then embedded in Durcupan ACM. The tomogram was generated from single tilt images that spanned -60 to 60° in 2° increments, acquired with a using an JEOL4000 IVEM. Magnification, 20000.0 X; accelerating voltage, 400 KeV.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
hippocampal neuron
Cellular Component
postsynaptic density
Bingren Hu
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
illumination by electrons
Visualization Methods
tungsten compound
Sample Preparation
glutaraldehyde fixed tissue
osmium tetroxide fixed tissue
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 492px ——
Y 492px ——