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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39969*  Cite 

Image mosaic of a section through mouse cerebellum showing the distribution of alpha synuclein (green) in a transgenic mouse overexpressing alpha synuclein. The section was counterstained with a nuclear stain (blue) to reveal the locations of cell somata. This image is a maximum projection of the Z-stack through the tissue, and has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

80µm thick sections were mounted in gelvitol using coverslips with a 1.0 µm thickness, sections were mounted in Vectashield with anit-fade. Images were acquired with a BioRad RTS 2000MP Multiphoton microscope using a Nikon 60X 1.4NA oil immersion objective. This mosaic image was reconstructed by manual alignment. For more information, see Price DL, Chow SK, MacLean NAB, Hakozaki H, Peltier S, Martone ME, Ellisman MH (2006) High-Resolution Large-Scale Mosaic Imaging using Multiphoton Microscopy to Characterize Transgenic Mouse Models of Human Neurological Disorders. Neuroinformatics. 2006;4(1):65-80.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Purkinje cell
cerebellar granule cell
Cellular Component
cell projection cytoplasm
cell body
neuronal cell body
Biological Context
Biological Process
cerebellum structural organization
Human Disease
Parkinson's disease
Diana Price
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
multi-photon microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
differences in adsorption or binding of stain
Visualization Methods
primary antibody plus labeled secondary antibody
Hoechst 33342
Alexa Fluor 488
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 800px 0.237µm
Y 615px 0.237µm