A single computed slice from a tomographic reconstruction of a lucifer yellow injected spiny dendrite from a striatal medium spiny neuron from the neostriatum of a dopamine transporter knock out mouse. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
Tissue from mouse strain: B6;129-/Slc6a3 tm2Mca, dopamine transporter knockout. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -70 to 70° in 2° increments. Magnification, 3000X, accelerating voltage, 3.0 MeV.
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