Computed slice through a tomographic reconstruction of the pre- and post-synaptic structures of the calycal synapse onto a chick ciliary ganglion neuron. This slice was derived from the 3rd of 6 serial volumes comprising the entire reconstruction. For more information, see: Shoop et al. Ultrastructure of a somatic spine mat for nicotinic signaling in neurons. J Neurosci. 2002 Feb 1;22(3):748-56. PMID: 11826104This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
Embryonic day (E) 15 chicks or 2-year-old adult chickens were perfusion-fixed with 2% paraformaldehyde plus 2% glutaraldehyde in cacodylate buffer, pH 7.4. Ciliary ganglia were removed, transferred to fresh fixative, and incubated for 3 hr at room temperature. Adult ciliary ganglia were cut into 1 mm3 pieces. After being rinsed several times in 0.1 M sodium cacodylate buffer, pH 7.4, the tissue was treated for 30 min with 2% osmium tetroxide in 0.1 M sodium cacodylate and then counterstained with uranyl acetate. The ganglia were dehydrated in a series of ethanol solutions followed by two rinses in acetone, infiltrated with Durcupan ACM resin (Electron Microscopy Sciences, Fort Washington, PA), allowed to polymerize for 24 hr at 60° C, and then sectioned. For serial tomography a continuous series of 10 1-µm-thick sections was prepared to encompass a complete spine mat. For traditional thin section analysis 100-nm-thick sections were made.
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