Electron micrograph of a cultured Drosophila DL1 cell infected with flock house virus, prepared using chemical fixation followed by routine embedding for electron microscopy. This specimen was prepared as a control to compare different high pressure freezing protocols. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
Cell pellets were conventionally prepared for electron microscopy by incubation in 2% glutaraldehyde in 100 mM cacodylate approx 5 min) and then on ice for 30 minutes, followed by 1 percent osmium tetroxide in double distilled water for 1 hour and 2 percent UA in water overnight. Images were acquired using a JEOL4000EX IVEM, magnification: 30000.0; accelerating voltage: 80.0 keV.
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