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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40234*  Cite 

Coronal section of rat brain triple-labeled with fluoromyelin (green), DiI (red), and TO-PRO3 (blue) which stain for myelin, blood vessels, and cell bodies, respectively. A maximum projection image was compiled by acquiring images across the entire rat brain section, and through 16 optical sections spanning the thickness of the section. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. It is also available through the Whole Brain Catalog (

Technical Details

The animal was perfused with Ringer's solution for 2 minutes followed by DiI for 10 minutes, and then 2% paraformaldehyde for 10 minutes. The brain was post-fixed overnight, and the tissue subsequently sectioned. After buffer washes, sections were stained with Fluoromyelin (1:100 overnight in cold room), rinsed with buffer washes, and subsequently stained TO-PRO3 (1:3000) for 45 minutes at room temp, rinsed and mounted using Gelvatol. 16 z-planes at 5 µm intervals across the entire section used to generate the tiled 3-D mosaic, acquired with an Olympus Fluoview 1000.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS neuron (sensu Vertebrata)
glial cell
blood vessel endothelial cell
Cellular Component
myelin sheath
Biological Context
Biological Process
central nervous system structural organization
Mark Ellisman
Stephen Larson
Sarah Maynard
Maryann Martone
Eric Bushong
CCDB 7290
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
compartmentalization of stain or label
Visualization Methods
fluorescent labels
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 389px ——
Y 512px ——