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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40440*  Cite 

Transmission electron micrograph of a vacuole from a sertraline-treated chc1 mutant cell. This vacuole shows evidence of active autophagy. This image is a high-magnification zoom of the cell in CIL 40415 (16,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448). The image contributor encourages Library users to add comments regarding possible causes the separation between the homogenous electron-opaque contents and the mixed opacity contents.

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Type
chc1 mutant
Cellular Component
autophagic vacuole
Biological Context
Biological Process
organelle organization
sertraline treatment
Ethan Perlstein
Daniel Korostyshevsky
Princeton EM Core Facility
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 2336px ——
Y 2300px ——