High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 fibroblast, showing the fiber-like structure. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 45 degrees, providing a pair that affords an oblique stereo view of the chromosome.
Cells in metaphase were exposed to 75 mM KCl, spread on water, picked up on grids, stained with uranyl acetate and critical point dried. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
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