High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 cell, showing the fibrous structure. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 45 degrees, providing a pair that affords an oblique stereo view of the chromosome.
Chromosomes were isolated from metaphase cells using a non-aqueous protocol, exposed to non-ionic detergent for 2 hr, then centrifuged through a sucrose cushion containing formaldehyde onto a carbon-coated grid (spreading technique of O.L. Miller), and critical point dried. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
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