Open Detailed Viewer

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40703^* Cite

Description

High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section of a Chinese hamster ovary cell in metaphase containing a longitudinal section of a chromatid pair, and revealing the fiber-like structure. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 45 degrees, providing a pair that affords an oblique stereo view of the chromosome.

Technical Details

Cells in metaphase were fixed, embedded in plastic, and 0.5 um sections cut and stained with uranyl acetate. See also: H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: epithelial cell
Cell Line: CHO
Cellular Component: nuclear chromosome

Biological Context

Biological Process: chromosome organization; mitosis; mitotic metaphase

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40703
Archival Resource Key (ARK): ark:/b7295/w9cil40703

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: chemically fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4792px	1nm
Y	6673px	1nm