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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:47363*  Cite 

Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 4 - Propidium iodide experiments)

Technical Details

Mice were anesthetized with ketamine/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized. GFP staphylococcus aureus beads were inserted. Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue). Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection.

Biological Sources
NCBI Organism Classification
Staphylococcus aureus
Cell Type
endothelial cell
Biological Context
Biological Process
cell death
Molecular Function
administration of isotype control abs
Mark Harding
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Source of Contrast
antibody labelling
Processing History
Converted from volocity to .avi
Sample Preparation
Relation To Intact Cell
intact cells-in vivo imaging
Spatial Axis Image Size Pixel Size
X —— 512µm
Y —— 512µm