Alpha-synuclein aggregates in the dendrites of primary neurons expressing the reconstituted miniSOG-Jα. α-syn aggregates contained in membrane-limited organelles, fusing to the plasma membrane suggesting release to the extracellular compartment.
Tomogram, 4 tilts. A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as “multicolor” electron microscopy (EM) via the photooxidation of 3-3′-diaminobenzidine and its derivatives.