Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50662*  Cite 
Description

Alpha-synuclein aggregates in the dendrites of primary neurons expressing the reconstituted miniSOG-Jα. α-syn aggregates contained in membrane-limited organelles, fusing to the plasma membrane suggesting release to the extracellular compartment.

Technical Details

Tomogram, 4 tilts. A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as “multicolor” electron microscopy (EM) via the photooxidation of 3-3′-diaminobenzidine and its derivatives.

Biological Sources
NCBI Organism Classification
Rat
Cell Type
cortical primary neurons
Cellular Component
dendrite
Attribution
Names
Daniela Boassa
Sebastien Phan
Junru Hu
John Ngo
Mark Ellisman
Link
Article
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL50662
Archival Resource Key (ARK)
ark:/b7295/w9cil50662
Imaging
Image Type
recorded image
Image Mode
Multi-tilt electron tomography